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KMID : 0385020040200020159
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Korean Journal of Laboratory Animal Science
2004 Volume.20 No. 2 p.159 ~ p.164
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Ethanol Model in Mongolian Gerbil for in vivo Comparison of Pathogenicity of Helicobacter Pylori Strains
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Lee Jin-Uk
Kim Ok-Jin
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Abstract
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The differences of pathogenicity associated with Helicobacter pylori (H. pylori) infection have not been well-defined between clinical isolates. This study was designed to establish ethanol model in Mongolian gerbil for in vivo comparison of pathogenicity of H. pylori strains. To determine an optimal ethanol concentration for ethanol model in gerbil, 5-week-old Mongolian gerbils were divided into different ethanol concentrations with or without HCl. After graded concentrations of ethanol, the macroscopic scores were measured with the stomach and duodenum of sacrificed animals. The macroscopic scores in the stomachs of sacrificed animals increased significantly in a concentration-dependent fashion. We determined an optimal ethanol concentration for ethanol model in gerbil as 60% and 100% without HCl and as 40% and 60% with 150 mM HCl. Thereafter, we evaluated whether this ethanol model in gerbil could be used as an useful tool for in vivo comparison of pathogenicity of H. pylori strains by enhancement of pathological severity in H. pylori-infected gerbils. Mongolian gerbils were divided into H. pylori-infected and PBS-inoculated groups. Thereafter, they were divided again into following subgroups-60% and 100% ethanol group and 40% and 60% ethanol with 150 mM HCl. After treatment, the severity of pathological changes was evaluated in a same manner with previous ethanol concentration-determining experiment. When the animals were exposed to ethanol, the gross scores were significantly higher in the infected group than in the uninfected group. These results suggested that the established gerbil-ethanol model in this study enhanced effectively the severity of pathogenic changes in the H. pylori-infected Mongolian gerbils and could be used as an useful tool for in vivo comparison of pathogenicity of H. pylori strains.
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KEYWORD
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Helicobacter pylori, Pathogenicity, Mongolian gerbil, Ethanol
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